On the 13th, the Ministry of Agriculture and Rural Affairs issued a work plan for the supervision of agricultural GMOs in 2020. It mentioned that the testing of genetically modified ingredients in the breeding materials and related breeding bases of seed companies should be conducted to prevent illegal genetically modified breeding. Conducted pull-up inspections on seed production bases, and carried out special inspection activities before spring plough preparation and seed harvest season.
So how do we detect invisible GMOs quickly and effectively? In order to reduce costs and expand the scope of monitoring, the Ministry of Agriculture recommends a test strip for rapid detection of GM. This small strip can detect the presence of GM components within 10-15 minutes, and can be used For the detection of plant seeds or leaf tissue of rice, corn and other plants.
high speed:
14 different rice and corn samples tested in 14 minutes
The shape, principle and usage of the genetically modified test paper are basically the same as those of the early pregnancy test paper. The test strip uses the principle of double antibody sandwich immunochromatography. During the lateral movement of the antigen in the sample, it collides with the specific monoclonal antibody 1 labeled with colloidal gold to form an antigen-antibody complex, which continues to flow forward and The binding of specific monoclonal antibody 2 on the NC membrane detection line forms a double antibody sandwich complex. If the antigen content in the sample is greater than 1%, the test line is red and the result is positive; otherwise, the test line is not colored and the result is negative.
low cost:
Test paper is about 10 yuan each, which is 40% of imported products, and the cost of auxiliary materials does not exceed 0.1 yuan.
Due to the different sensitivity and specificity of different test strips, general test strips need to be tested with a special extraction solution to avoid false negatives caused by insufficient protein release and false positives caused by too much chlorophyll. "And our test strips are more 'skinned', and the test water is pure water or even tap water."
Accuracy:
Improper operation can also cause various problems such as false positives, false negatives, and poor flow
The rapid test strip is one of the very few products that has been validated on a large scale to test polished rice. The polished rice has a very low protein content and contains interfering ingredients such as polishing agents. The test strip effectively solves the problem of false negatives and false positives common in similar products, and the test results are accurate. In addition, the test strip can also be used for the detection of deep-processed products such as corn starch, corn dextrin, and rice protein, with good results.
The general method of transgenic detection is PCR qualitative screening test. The test cost is about 1,000 yuan each time, while the test strip test cost is about 10.1 yuan. So can 10.1 yuan solve the problem of 1,000 yuan? Even if the test strip test result is positive, it is still in the "sea selection" stage. It is still necessary to send the "sea selection" contestants to a professional institution for PCR qualitative screening and testing to finally determine whether they actually contain genetically modified materials. "But just a few thousand yuan can be saved in the early stages."
There are several types of genetically modified test papers. What conditions need to be met?
Taking the top genetically modified test paper of TOP Yunong as an example, it is mainly divided into insect-resistant test paper, herbicide-resistant test paper, and insect-resistant kit. It has the advantages of strong specificity, high sensitivity, wide versatility, fast response speed, and storage resistance .
Anti-insect test strips and anti-insect kits are mainly used for the rapid detection of genetically modified Bt Cry1AB / 1Ac, BtCry1C, Bt Cry2A in samples such as seeds or plant leaves, with a sensitivity of 1%. The entire detection process only takes 10-15 minutes and is suitable for various types Enterprises and testing agencies. The test line (T line) is red and the result is positive: otherwise, the test line (T line) is not colored and the result is negative. It can be used to detect plant seeds or plant leaf tissues such as rice and corn.
Herbicide-resistant test strips are mainly used to quickly detect genetically modified CP4EPSPS in samples such as plant cereals and bulk grains, with a sensitivity of 0.2%. This detection process only takes 10 minutes and is suitable for various enterprises and testing institutions. If the amount of bulk genetically modified grains in the sample is greater than 0.2%, the detection line is red, and the result is a positive line; otherwise, the detection line is not colored, and the result is negative.
Overview of genetically modified products
Test strip name
specification
Test sample
GMO rapid test strip (anti-insect test strip)
Bt Cry1AB / Ac
100 pieces / box
Rice / wheat / cotton seed leaves
Bt Cry2A
100 pieces / box
Rice / wheat / cotton seed leaves
Bt Cry1c
100 pieces / box
Rice / wheat / cotton seed leaves
GMO rapid test strip (herbicide-resistant strip)
CP4EPSPS
100 pieces / box
Mainly detect soybean, corn, etc.
BAR (import)
100 pieces / box
Mainly detect soybean, corn, etc.
BAR (domestic)
100 pieces / box
Mainly detect soybean, corn, etc.
Crop Genetically Modified Kit (Anti-insect Kit)
Bt Cry1AB / Ac
Kit 96 wells
Rice / wheat / cotton seed leaves
Bt Cry2A
Kit 96 wells
Rice / wheat / cotton seed leaves
Bt Cry1c
Kit 96 wells
Rice / wheat / cotton seed leaves
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